Comparative Proteomics of E. Coli O157:H7: Two-Dimensional Gel Electrophoresis vs. Two-Dimensional Liquid Chromatography Separation~!2010-03-15~!2010-06-09~!2010-09-09~!

Comparative Proteomics of E. Coli O157:H7: Two-Dimensional Gel Electro- phoresis vs. Two-Dimensional Liquid Chromatography Separation

The method for comparing bacterial proteomes has traditionally been two-dimensional gel electrophoresis (2-D GE); however, in recent years, new procedures for protein separation have been introduced. One of these new procedures utilizes column-based liquid chromatography (2-D LC) separation. The techniques by which these two methods separate proteins differ significantly; however, it is current...

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

The pioneering work by Patrick H. O'Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry1975, 250, 4007-4021). The application of two-dimensional gel electrophoresis has played a key role in the systematic identification and detailed characterization of the prote...

Two Dimensional Gel Electrophoresis in Cancer Proteomics

Two-dimensional electrophoresis (2-DE) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique sort’s protein according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their isoelectric points (pI); the se...

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis and the beginning of proteomics.

Featured article: O’Farrell PH. High resolution twodimensional electrophoresis of proteins. J Biol Chem 1975;250:4007–21. No one had heard of proteomics in 1971. The invention of the word was 25 years away (1 ). At the time, doing molecular biology meant studying bacteria or their viruses, the bacteriophage. But as a starting graduate student in Boulder, Colorado, I wanted to study eukaryotic e...